Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.
Upon delivery store at -70☌.įor Use in Research only. Prepare aliquots immediately after reconstitution for deep freezing.Īt ambient temperature. Store on ice after reconstitution and use final mixtures within 1 day. After reconstitution aliquots of FluMaXx, Sol. B).Īll components were made up in Milli-Q™ water and 0.2µm filtered.įluMaXx is stored at -70☌ upon arrival and will be stable in performance for at least 6 months from the date of purchase. It is recommended to shortly degas FluMaXx before use to remove trapped air from the viscous solution.Īs antifading buffer system: add 450µl of ultrapure water to the tube labelled Sol. B (ATP/DTT-Mix) is added freshly before mixing with the sample solution to be observed (e.g. A, the mixture is vortexed and centrifuged for 1min, 5000rpm. A and mix with 200µl of methylcellulose (MC). After addition of the MC-solution to Sol.
(Contains methylcellulose as 0.5% solution)Ĭontains: Glucose oxidase, catalase, imidazole pH 7.4, KCl, EGTA, MgCl2, glucose, cryoprotectants (proprietary formula) ġx 1.5ml Methylcellulose stock solution (0.5% in Milli-Q™ water)įor flow chamber assays: add 250µl of ultrapure water to the tube labelled Sol. Lyophilized, three-component buffer system, ready-to-use. To guarantee the high quality of FluMaXx, all critical components are supplied as a lyophilized powder.
The buffer contains 5mM DTT, showing a beneficial effect on performance without measurable damage of the enzyme system. Our proprietary formula of the FluMaXx permits refreezing after reconstitution without loss of activity. Oxygen scavengers are a technology that extends the shelf life and maintains the quality and freshness of food products. Catalase normally cannot be frozen in solution without a major loss of activity and furthermore is inactivated by higher concentrations of reducing agents. filamentous actin from fragmentation, which are extremely prone to photon induced chemical damage.įluMaXx contains all vital components for high resolution monitoring of assays using cytoskeletal polymers. This optimized catalase/glucose oxidase enzyme system, protects the natural fluorescence intensity of the dye and thus maXximizes the fluorescence intensity.Ī novel optimization of FluMaXx is based on the stabilization of the catalyse enzyme. The efficiency of FluMaXx is illustrated by its ability to prevent e.g. Oxygen scavenging system for single fluorescent molecule imaging.įlow chamber protein interaction assays, all applications demanding strong antifade supplements, Suited for all types of fluorescence microscopy and spectroscopical applications and methods: TIRFM, Epifluorescence microscopy (EFM), GSD, STED, dStorm etc.įluMaXx is an fluorescence oxygen scavenging system to improve the stability of fluorescent dyes.
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